How The CD44 Antibody Can Help Your Research

by | Feb 20, 2017 | Health

The CD44 antibody is a glycoprotein on the cell surface, expressed on monocytes, granulocytes, and lymphocytes. It is considered a homing molecule for cell adhesion, as well as ECM-III, Phagocytic glycoprotein-1, and Hermes-1/HUTCH-1. It’s been implicated in cell migration, tumor metastasis, and lymphocyte homing. It was designed to be used for research, which means you can use it to learn more about particular problems in a variety of areas of the body. It has a clone of SPM 521, as well.


It is designed for use in Western Blotting and Immunohistochemistry. The CD44 antibody has a molecular weight of 80kDa.

The positive control for IHC applications is the esophageal carcinoma. The procedure is to start with preparation of the specimen using paraffin-embedded or Formalin-fixed tissues. Deparaffinized slides should be used at all times. When using the concentrated version of the formula, you should dilute using a ratio of 1 to 50, and you should always dilute according to package directions or purchase pre-diluted formulas.

To retrieve the antigen for further testing, you will need to boil your section of tissue in a citrate buffer with a pH of 6.0 for at least 10 minutes, allowing it to cool to room temperature for 20. Incubation periods are 30 minutes at room temperature.

The positive control for Western Blotting applications is the HeLa cell lysate with a cellular localization in the membrane. It is recommended that you dilute the CD44 antibody with a ratio of 1:25. Incubation periods are one hour at room temperature for most applications.

You can find a 7.0 ml pre-diluted formula, as well as 0.1, 0.5, and 1.0 ml options that will need to be diluted accordingly.

The CD44 antibody can be used in a variety of ways, researching various things. Visit Spring Bioscience to learn more and purchase yours today.

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