The Anti-CD3 antibody is designed to react with the antigen of the same name in the intracytoplasmic portion expressed by T-cells. It can stain human T-cells in the medulla and cortex of the thymus, as well as in the peripheral lymphoid tissues. It is suitable to stain both neoplastic and normal T-cells, as well.
More Information
While you can’t use this antibody for diagnostics, it is perfect for use in research. It has no available clone, and the immunogen is the synthetic 13-mer peptide that corresponds to the internal region of the human protein of the same name in the epsilon chain. The isotype is the Rabbit IgG, and it has an undetermined epitope with a molecular weight of 19kDa.
Applications
The Anti-CD3 antibody can be used for Western Blotting and Immunohistochemistry applications.
The IHC procedure involves using paraffin-embedded or Formalin-fixed tissues, as well as deparaffinized slides. You should dilute the antibody using a ratio of 1:200, though dilutions are estimates. You can also find a pre-diluted formula that can work well in this situation.
To retrieve the antigen, you should boil tissue sections in a Citrate buffer with a pH of 6.0 for at least ten minutes, and then allow it to cool for 20 minutes. Incubate the concoction for another ten minutes at room temperature.
For Western Blotting, you should dilute the antibody using a ratio of 1:25, which means you cannot use the pre-diluted formula. Incubate it for one hour at room temperature.
The positive control for IHC is the tonsil with the Jurkat cell lysate being the positive control for Western Blotting. Both versions have cellular localization occurring in the membrane.
The Anti-CD3 is an excellent research tool for a variety of tissue samples. Visit Spring Bioscience at for more information on how to purchase some for your lab.
